pelleted: Calculate the fraction pelleted for a vesicle size

In wfondrie/evrspin: Calculations for Extracellular Vesicle Sedimentation by Centrifugation

Description

Calculates the fraction pelleted for a vesicle of a specified diameter and rcf for a specified time. Units are noted in parentheses.

Usage

pelleted(t, d, rcf, rotor, vesicle.density = 1.15, solvent.density = 1,
  viscosity = 0.0155)

Arguments

t	The centrifugation time (min).
d	The vesicle diameter (nm).
rcf	The relative centrifugal force.
rotor	A rotor object, created with rotor().
vesicle.density	The vesicle density (g/cm^3).
solvent.density	The solvent density (g/cm^3).
viscosity	The viscosity of the solvent (g/(cm*s)).

Details

Calculates the fraction of a vesicle population that is pelleted during a cetrifugation run at the specified time and rcf. Though the original equation allows for fractions greater than 1 (for swinging bucket rotors) or may become undefined (for fixed-angle rotors) the maximum value pelleted() will return is 1 due to the physical meaning of 100

The default value for vesicle.density is 1.15 g/cm^3, which is the most common exosome density in the literature.

The default solvent.density is 1.00 g/cm^3 and the default viscosity is 0.0155 g/(cm*s), which are respectively the density and viscosity of pure water.

Value

The fraction of a vesicle population pelleted. Is always numeric between [0, 1].

Examples

# create a Beckman SW 40 Ti rotor object
sw40 <- rotor("sw40")

pelleted(t = 60, d = 100, rcf = 10000, sw40)
pelleted(t = 60, d = 100, rcf = 100000, sw40)


# Arguments can be of length > 1
pelleted(t = 1:100, d = 100, rcf = 10000, sw40)
pelleted(t = 60, d = 100:110, rcf = 10000, sw40)
pelleted(t = 60, d = 100, rcf = seq(100, 1000, by = 100), sw40)
pelleted(t = 1:10, d = 100:110, rcf = seq(100, 1000, by = 100), sw40)

wfondrie/evrspin documentation built on May 4, 2019, 5:22 a.m.