sed_time: Calculate the time to completely sediment a vesicle

Description Usage Arguments Details Value Examples

View source: R/sed_time.R

Description

Calculates the time needed for complete sedimentation of vesicles with specified size and rcf. Units are noted in parentheses.

Usage

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sed_time(d, rcf, rotor, vesicle.density = 1.15, solvent.density = 1,
  viscosity = 0.0155)

Arguments

d

The vesicle diameter (nm).

rcf

The relative centrifugal force.

rotor

A rotor object, created with rotor().

vesicle.density

The vesicle density (g/cm^3).

solvent.density

The solvent density (g/cm^3).

viscosity

The viscosity of the solvent (g/(cm*s)).

Details

Calculates the centrifugation time needed to completely sediment a vesicle population at the provided rcf. This is the theoretical maximum time for a vesicle to traverse the sedimentation path length or sed.L of a rotor object.

The default value for vesicle.density is 1.15 g/cm^3, which is the most common exosome density in the literature.

The default solvent.density is 1.00 g/cm^3 and the default viscosity is 0.0155 g/(cm*s), which are respectively the density and viscosity of pure water.

Value

The time to completely sediment a vesicle population (min).

Examples

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# create a Beckman SW 40 Ti rotor object
sw40 <- rotor("sw40")

sed_time(d = 100, rcf = 10000, sw40)
sed_time(d = 100, rcf = 100000, sw40)


# Arguments can be of length > 1
sed_time(d = 100:110, rcf = 10000, sw40)
sed_time(d = 100, rcf = seq(100, 1000, by = 100), sw40)
sed_time(d = 100:110, rcf = seq(100, 1000, by = 100), sw40)

wfondrie/evrspin documentation built on May 4, 2019, 5:22 a.m.