steadyState: Analysis of steady state fluorescence flow cytometry

In wrightrc/flowTime: Annotation and analysis of biological dynamical systems using flow cytometry

Analysis of steady state fluorescence flow cytometry

Description

Generates a data frame which can be used to visualize and analyze steady state flow cytometry data. Steady state in this case means that

Usage

steadyState(flowset, gated = FALSE, ploidy = NA, only = NA)

Arguments

flowset	your flowSet to be analyzed
gated	boolean is the data already gated?
ploidy	character gate to subset your flowset based on the ploidy of you strains
only	character which population of events to analyze, 'yeast', 'singlets', or 'doublets'?

Value

a data frame containing all of the selected subset of events from the original flowSet

Examples

dat <- read.flowSet(path = system.file("extdata", "ss_example",
package = "flowTime"), alter.names = TRUE)
annotation <- read.csv(system.file("extdata", "ss_example.csv",
package = "flowTime"))
dat <- annotateFlowSet(dat, annotation, mergeBy = "name")
loadGates(gatesFile = 'SORPGates')
steadyState(dat, gated = FALSE, ploidy = "diploid", only = "singlets")

wrightrc/flowTime documentation built on Dec. 8, 2022, 9:50 p.m.