read.qPCRtable: read qPCR data table with Ct values. See "Details".
In zgzhao/Xtools: Xtools: handy tools for daily data analysis

Description Usage Arguments Details Value Author(s)

Read qPCR Ct data

1	read.qPCRtable(fname, na.value = 40, ...)

`fname`	file name (path to Ct data file)
`na.value`	Number. If Ct is NA or greater than this value, set to this value.
`...`	Parameters passed to read.table function.

Ct values are exported from qPCR machine. Ct values should have labels of well index, gene name, sample tags and repeat number. Arrange of data: - save data in csv or tab-delim format - required columns: "Well", "Ct", "gene" and "rep.num" - sample labels (required) can be put in a single "sample" column, or presented in multi columns named with "ds.xxxx" pattern. - duplicated well label ("Well" column) are not allowed - all samples should have tested same list of genes of identical repeats. - avoid using space and other special character in column names.

list of data.ht (qPCRset) and data.ddct (ddCt) objects.

ZG Zhao

zgzhao/Xtools documentation built on May 22, 2021, 3:37 a.m.