title: "RNA-seq 2G" author: "Zhe Zhang" date: "2016-09-26" output: html_document: number_sections: yes self_contained: no toc: yes toc_float: collapsed: no
Two-group comparison of differential expression (DE) is the most common analysis of transcriptome data. For RNA-seq data, the comparison is usually performed on a gene-level matrix of read counts, with the read counts corresponding to the number of sequencing reads mapped to each gene in each RNA-seq sample.
Statistical methods that have been applied to two-group DE of RNA-seq data are widely different in terms of their data distribution assumption, input/output format, performance, sensitivity, and user-friendliness.
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