flu_norm: Normalise fluorescence against negative well

In ucl-cssb/flopr: A package for the normalisation and calibration of flow cytometry and plate reader spectrophotometry data

Normalise fluorescence against negative well

Description

Normalise fluorescence against negative well

Usage

flu_norm(pr_data, neg_well, blank_well, flu_name, af_model, data_csv)

Arguments

pr_data	a long data.frame containing you plate reader data with OD normalised
neg_well	the well coordinates of a non-fluorescent control
blank_well	the well coordinates of a media blank
flu_name	the column name of the fluorescence chanel to normalise
af_model	model used to fit negative control autofluorescence. For now these include "polynomial", "invers_poly", "exponential", "spline" or "loess".
data_csv	path to the original data. Used for saving normalisation curve plots.

ucl-cssb/flopr documentation built on Jan. 27, 2024, 11:49 a.m.