quantifyPSI: AS event quantification

In ASpediaFI: ASpedia-FI: Functional Interaction Analysis of Alternative Splicing Events

Description

Compute PSI values of AS events. This function borrows code from the IMAS package.

Usage

quantifyPSI(object, read.type = "paired", read.length, insert.size,
    min.reads, num.cores = 1)

Arguments

object	Object of class ASpediaFI
read.type	a type of RNA-seq reads ('single' or 'paired')
read.length	read length
insert.size	insert size
min.reads	a minimum number of reads mapped to a given exon
num.cores	the number of cores for parallel processing

Value

ASpediaFI object with PSI values

References

Han, S. et al. (2017). IMAS: Integrative analysis of Multi-omics data for Alternative Splicing. R package version 1.8.0.

Examples

bamWT <- system.file('extdata/GSM3167290.subset.bam', package = 'ASpediaFI')
GSE114922.ASpediaFI <- ASpediaFI(
    sample.names = 'GSM3167290',
    bam.files = bamWT, conditions = 'WT'
)
## Not run: 
GSE114922.ASpediaFI <- quantifyPSI(GSE114922.ASpediaFI,
    read.type = 'paired',
    read.length = 100, insert.size = 300,
    min.reads = 3, num.cores = 1
)

## End(Not run)

ASpediaFI documentation built on Nov. 8, 2020, 8:13 p.m.