estimate_IG_indicators: Estimate the intrinsic gene indicators

In BUScorrect: Batch Effects Correction with Unknown Subtypes

Description

Call the function to estimate the intrinsic gene indicators.

Usage

estimate_IG_indicators(BUSfits, postprob_DE_threshold = 0.5)

Arguments

BUSfits	The BUSfits object output by the function BUSgibbs.
postprob_DE_threshold	the threshold to call an intrinsic gene indicator to be one or not according to whether its posterior probability is higher than postprob_DE_threshold or not. The default is 0.5.

Value

est_L

the estimated intrinsic gene indicators, a matrix where the rows represent genes and the columns correspond to subtypes k=2,...,K

Author(s)

Xiangyu Luo

References

Xiangyu Luo, Yingying Wei. Batch Effects Correction with Unknown Subtypes. Journal of the American Statistical Association. Accepted.

Examples

rm(list = ls(all = TRUE))  
set.seed(123)
#a toy example, there are 6 samples and 20 genes in each batch
example_Data <- list()

#batch 1
example_Data[[1]] <- rbind(matrix(c(1,1,5,5,10,10,
						3,3,7,7,12,12), ncol=6, byrow=TRUE), matrix(c(1,2),nrow=18, ncol=6))

#batch 2
batch2_effect <- c(2,2,2,1,1)
example_Data[[2]] <- rbind(matrix(c(1,1,5,5,10,10,
						3,3,7,7,12,12), ncol=6, byrow=TRUE), matrix(c(1,2),nrow=18, ncol=6)) + batch2_effect

#batch 3
batch3_effect <- c(3,2,1,1,2)
example_Data[[3]] <- rbind(matrix(c(1,1,5,5,10,10,
						3,3,7,7,12,12), ncol=6, byrow=TRUE), matrix(c(1,2),nrow=18, ncol=6)) + batch3_effect

set.seed(123)
BUSfits <- BUSgibbs(example_Data, n.subtypes = 3, n.iterations = 100, showIteration = FALSE)
#select the posterior probability threshold to estimate the intrinsic gene indicators
thr0 <- postprob_DE_thr_fun(BUSfits, fdr_threshold=0.1)
est_L <- estimate_IG_indicators(BUSfits, postprob_DE_threshold=thr0)

#obtain the intrinsic gene indicators
intrinsic_gene_indices <- IG_index(est_L)

BUScorrect documentation built on Nov. 8, 2020, 8:06 p.m.