The package offers a function to create DNA barcode sets capable of correcting substitution errors or insertion, deletion, and substitution errors. Existing barcodes can be analysed regarding their minimal, maximal and average distances between barcodes. Finally, reads that start with a (possibly mutated) barcode can be demultiplexed, i.e. assigned to their original reference barcode.
create.dnabarcodes creates a set of barcodes of
equal length that satisfies some wished criteria regarding error correction.
After sequencing the DNA/RNA material, the researcher will have a set of reads
that start with a (possibly mutated) barcode. For Illumina HiSeq, this is the
index read. For PacBio, this is the read itself (with some other
complications). The function
demultiplex can then be used to
assign reads to their original reference barcodes.
will correct mutations in a best-effort way.
Existing sets of barcodes (e.g. supplied by a manufacturer) can be analysed
The advantage of this package over using already available barcode sets in the
scientific community is the ability to flexibly generate new barcode sets of
different properties. For example,
create.dnabarcodes can use a
pre-existing barcode library as a candidate set for a better barcode set. In
another example, a higher distance (e.g.,
dist = 4) can be used. Such a
parameter setting would possibly increase the error detection property of the
code as well as the average barcode distance, increasing the probability of
guessing a barcode during demultiplexing.
Tilo Buschmann ([email protected])
Buschmann, T. and Bystrykh, L. V. (2013) Levenshtein error-correcting barcodes for multiplexed DNA sequencing. BMC bioinformatics, 14(1), 272. Available from http://www.biomedcentral.com/1471-2105/14/272.
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