positiveFactor: Acessor functions for the 'positiveFactor' slot in a...
In NanoStringDiff: Differential Expression Analysis of NanoString nCounter Data

Description Usage Arguments Details Value Author(s) See Also Examples

Positive size factors can be used to adjust all platform associated sources of variation.

## S4 method for signature 'NanoStringSet'
positiveFactor(object)
## S4 replacement method for signature 'NanoStringSet,numeric'
positiveFactor(object) <- value

`object`	A NanoStringSet object.
`value`	A vector of positive size factors.

The observed counts including negative control genes and housekeeping control genes might be effect by some experimental factors like hybridization and binding efficiency. In order to get the true rate of gene expression, these variations must be normalized. Positive size factors can normalize this kind of variation.

A vector contain positive size factors

Hong Wang <hong.wang@uky.edu> chi wang <chi.wang@uky.edu>

positiveControl

data(NanoStringData)
## obtain positive factors
positiveFactor(NanoStringData)

## assign a vector
n=ncol(exprs(NanoStringData))
positiveFactor(NanoStringData)=rep(1,n)