(the Sassolungo mountain in the Dolomites)
alpine is an R/Bioconductor package for modeling and correcting fragment sequence bias for RNA-seq transcript abundance estimation. In particular, it is the first method of its kind to take into account sample-specific dependence of RNA-seq fragments on their GC content.
An example workflow can be found in
vignettes/alpine.Rmd, or by typing:
alpine is designed for un-stranded paired-end RNA-seq data.
A paper explaining the alpine methods was published in December 2016:
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