SDMFrame: Read an SDM file

In ddCt: The ddCt Algorithm for the Analysis of Quantitative Real-Time PCR (qRT-PCR)

Description

Read an SDM file: Data Output File for SDS, Version 2.1

Usage

  SDMFrame(file)
  readSDM(file)

Arguments

file	Character vector of filenames

Details

This function reads the data given in the colums 'Detector','Sample' and 'Ct' of the specified SDM output file(s) and stores them as a data.frame. An additional column including the respective filename is added.

This function is a wrapper for the SDMFrame constructor

Value

A object of class SDMFrame

Author(s)

Rudolf Biczok mailto:[e-mail]

Examples

## read a SDM file
sampdat <- SDMFrame(system.file("extdata", "Experiment1.txt",
                                package="ddCt"))
## you can also write 
## sampdat <- new("SDMFrame",system.file("extdata", "Experiment1.txt",
##                                       package="ddCt"))

## or with
## sampdat <- readSDM(system.file("extdata", "Experiment1.txt",
##                                package="ddCt"))

## use the getter methods
sampleNames(sampdat)

## or the overloaded primitive accessors
sampdat[1:3,"Sample"]

## see all unique samples
uniqueSampleNames(sampdat)

## replace all sample names 'Sample1' and 'Sample2' in sampdat
## with 'NewSample1' and 'NewSample2'
uniqueSampleNames(sampdat,c("Sample1","Sample2")) <- c("NewSample1","NewSample2")
uniqueSampleNames(sampdat)

## or use this syntax to replace the gene names
uniqueDetectorNames(sampdat) <- c(Gene1="NewGene1", Gene2="NewGene2")
uniqueDetectorNames(sampdat)

ddCt documentation built on Nov. 8, 2020, 4:57 p.m.