This method finds a split in the low density region of the provided object (which shall be either a matrix or flowCore's flowFrame object) and returns a vector of TRUE/FALSE depending on whether each of the events (rows) are left or righ (i.e, lower or higher) than density peak. An FCS channel shall be specified if a flowFrame object with multiple channels in provided on the input. If matrix is the input then it shall contain one column only. This method is designed to work well for 2 level beads, such as stained and unstained, but will not provide meaningful results for multi-level beads or other data in general.
Object of class
Additional options, see the details section.
Additional parameter of the method:
Which FCS channel shall be used in order to look for the split in the data? This is applicable if a flowFrame object with several channels is used on the input.
A vector of TRUE/FALSE values depending on whether each of the events (rows) are below or above the identified split value.
Wayne Moore, Faysal El Khettabi, Josef Spidlen
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library('flowCore') library('flowQBData') fcsFilePath <- system.file("extdata", "SSFF_LSRII", "Other_Tests", "933745.fcs", package="flowQBData") myFlowFrame <- read.FCS(fcsFilePath) ## Note that this is just to demonstrate the syntax, but doing this on the ## FSC and SSC channels of this particular FCS file is not very meaningful r1 <- split_in_two(myFlowFrame, 'FSC-H') r2 <- split_in_two(exprs(myFlowFrame[,'SSC-H']))
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