Description Usage Arguments Examples
Create relative log expression (RLE) plot from ExpressionSet. RLE plots were initially proposed to measure the overall quality of a dataset but can also be used to visualize the presence of unwanted batch effects in the data.
1 |
eset |
ExpressionSet object. |
colLabel |
colname in pData(eset) to retrieve information for the labeling of samples with a color. All samples with the same value in pData(eset)[,colLabel] will share the same color. |
legend |
If TRUE a legend will be provided next to the RLE plot. |
file |
If defined, the resulting plot will be stored as a pdf file instead of shown interactively. |
... |
Additional parameters for the 'plot' function (e.g. 'main'). |
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 | # retrieve two datasets:
library(inSilicoDb);
InSilicoLogin("rpackage_tester@insilicodb.com", "5c4d0b231e5cba4a0bc54783b385cc9a");
eset1 = getDataset("GSE18842", "GPL570", norm="FRMA", features="gene");
eset2 = getDataset("GSE31547", "GPL96", norm="FRMA", features="gene");
esets = list(eset1,eset2);
# merge them using no additional merging technique and the 'COMBAT' method:
library(inSilicoMerging)
eset_FRMA = merge(esets);
eset_COMBAT = merge(esets, method="COMBAT");
# check available annotations:
colnames(pData(eset_FRMA))
table(pData(eset_FRMA)[,"Disease"]);
table(pData(eset_FRMA)[,"Study"]);
# Visual inspection of the two merged datasets through an MDS plot
plotRLE(eset_FRMA, colLabel="Disease")
plotRLE(eset_COMBAT, colLabel="Disease")
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