normalize450K: Preprocessing of Illumina Infinium 450K data

Precise measurements are important for epigenome-wide studies investigating DNA methylation in whole blood samples, where effect sizes are expected to be small in magnitude. The 450K platform is often affected by batch effects and proper preprocessing is recommended. This package provides functions to read and normalize 450K '.idat' files. The normalization corrects for dye bias and biases related to signal intensity and methylation of probes using local regression. No adjustment for probe type bias is performed to avoid the trade-off of precision for accuracy of beta-values.

Package details

AuthorJonathan Alexander Heiss
Bioconductor views DNAMethylation MethylationArray Microarray Normalization Preprocessing TwoChannel
MaintainerJonathan Alexander Heiss <>
LicenseBSD_2_clause + file LICENSE
Package repositoryView on Bioconductor
Installation Install the latest version of this package by entering the following in R:

Try the normalize450K package in your browser

Any scripts or data that you put into this service are public.

normalize450K documentation built on April 29, 2020, 2:17 a.m.