Gene expression is a stochastic process, which often results in substantial
expression noise. To obtain a putative set of transcribed regions, it is
necessary to identify those regions that are expressed significantly above
the background level. Using a Poisson-based approach for estimating
the noise distribution from the frequency of the transcribed regions with the
low fragments coverage,
estimateBackground function returns a
coverage cutoff value for a specific
False Discovery Rate (FDR).
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of the provided
TranscriptionDataSet object will be updated by
the FDR cutoff value used in the calculations and by the corresponding
estimated coverage cutoff value, respectively.
Armen R. Karapetyan
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