DetectEvents.AFE: Detect alternative first exons

In MARVEL: Revealing Splicing Dynamics at Single-Cell Resolution

Detect alternative first exons

Description

Detects alternative first exons from GTF. This is a wrapper function for DetectEvents.AFE.PosStrand and DetectEvents.AFE.NegStrand functions.

Usage

DetectEvents.AFE(
  MarvelObject,
  min.cells = 50,
  min.expr = 1,
  track.progress = FALSE
)

Arguments

MarvelObject	Marvel object. S3 object generated from CreateMarvelObject function.
min.cells	Numeric value. The minimum number of cells in which the gene is expressed for the gene to included for splicing event detected and quantification. To be used in conjunction with min.expr argument. Default value is 50.
min.expr	Numeric value. The minimum expression value for the gene to be considered to be expressed in a cell. Default value is 1.
track.progress	Logical. If set to TRUE, progress bar will appear to track the progress of the rate-limiting step of this function, which is the extraction of the final exon-exon junctions. Default value is FALSE.

Value

An object of class S3 with new slot MarvelObject$SpliceFeature$AFE.

Examples

marvel.demo <- readRDS(system.file("extdata/data", "marvel.demo.rds", package="MARVEL"))

marvel.demo <- DetectEvents.AFE(MarvelObject=marvel.demo,
                                min.cells=5,
                                min.expr=1,
                                track.progress=FALSE
                                )

MARVEL documentation built on Oct. 31, 2022, 5:07 p.m.