NanoString nCounter data are gene expression assays where there is no need for the use of enzymes or amplification protocols and work with fluorescent barcodes (Geiss et al. (2018) <doi:10.1038/nbt1385>). Each barcode is assigned a messenger-RNA/micro-RNA (mRNA/miRNA) which after bonding with its target can be counted. As a result each count of a specific barcode represents the presence of its target mRNA/miRNA. 'NACHO' (NAnoString quality Control dasHbOard) is able to analyse the exported NanoString nCounter data and facilitates the user in performing a quality control. 'NACHO' does this by visualising quality control metrics, expression of control genes, principal components and sample specific size factors in an interactive web application.
Package details |
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Author | Mickaël Canouil [aut, cre] (<https://orcid.org/0000-0002-3396-4549>), Roderick Slieker [aut] (<https://orcid.org/0000-0003-0961-9152>), Gerard Bouland [aut] |
Maintainer | Mickaël Canouil <pro@mickael.canouil.dev> |
License | GPL-3 |
Version | 2.0.6 |
URL | https://github.com/mcanouil/NACHO/ https://m.canouil.dev/NACHO/ |
Package repository | View on CRAN |
Installation |
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