new-method: Creates new instance of 'RDML' class object

In RDML: Importing Real-Time Thermo Cycler (qPCR) Data from RDML Format Files

Creates new instance of RDML class object

Description

This function has been designed to import data from RDML v1.1 and v1.2 format files or from xls file generated by Applied Biosystems 7500. To import from xls this file have to contain Sample Setup and Multicomponent Data sheets!

Arguments

filename	string – path to file
show.progress	logical – show loading progress bar if TRUE
conditions.sep	separator for condition defined at sample name
format	string – input file format. Possible values auto, rdml, abi, excel, csv. See Details.

Details

File format options:

auto

Tries to detect format by extension. .xlsx – excel, .xls – abi, .csv – csv, other – rdml

abi

Reads .xls files generated by ABI 7500 v.2. To create such files use File>Export; check 'Sample Setup' and 'Multicomponent Data'; select 'One File'

excel

.xls or .xslx file with sheets 'description', 'adp', 'mdp'. See example file table.xlsx

csv

.csv file with first column 'cyc' or 'tmp' and fluorescence data in other columns

rdml

.rdml or .lc96p files

Warning

Although the format RDML claimed as data exchange format, the specific implementation of the format at devices from real manufacturers differ significantly. Currently this function is checked against RDML data from devices: Bio-Rad CFX96, Roche LightCycler 96 and Applied Biosystems StepOne.

Author(s)

Konstantin A. Blagodatskikh <[e-mail]>, Stefan Roediger <[e-mail]>, Michal Burdukiewicz <[e-mail]>

Examples

## Not run: 
## Import from RDML file
PATH <- path.package("RDML")
filename <- paste(PATH, "/extdata/", "lc96_bACTXY.rdml", sep ="")
lc96 <- RDML$new(filename)

## Some kind of overview for lc96
lc96$AsTable(name.pattern = sample[[react$sample$id]]$description)
lc96$AsDendrogram()

## End(Not run)

RDML documentation built on Aug. 9, 2025, 1:07 a.m.