Man pages for TmCalculator
Genome-Wide Nucleic Acid Melting Temperature Profiling and Multi-Omics Integration

cash-.TmCalculatorLazy '$df' accessor for 'TmCalculator' objects
check_filter_seqFilter invalid bases in nucleotide sequences
chem_correctCorrections of melting temperature with chemical substances
compare_groupsCompare numeric GRanges metadata across groups
complement_fastFast complement and reverse complement
coor_to_genomic_rangesConvert genomic coordinate strings to a GRanges object
dot-chem_correct_vecVectorized chemical correction over per-sequence GC percent
dot-fasta_lengthsRecord lengths of a FASTA file, without reading the sequences
dot-filter_N_windowsFilter windows with too many N bases
dot-find_N_boundsDetect the first and last non-N positions on a chromosome
dot-gc_vecVectorized GC percent over a character vector of sequences
dot-get_bsgenome_from_pkgLoad the BSgenome object from an installed BSgenome.* data...
dot-getseq_preload_chrSequence extraction by preloading whole chromosomes
dot-getseq_vectorizedVectorized sequence extraction with getSeq
dot-load_genome_packagesLoad installed BSgenome packages
dot-normalize_tm_gc_metadataNormalize Tm/GC metadata column names on a GRanges object
dot-parse_coord_stringsParse coordinate strings into a data frame
dot-salt_correct_vecVectorized salt correction over per-sequence GC percent and...
dot-spill_fastaStage sequences as a FASTA file so that workers read rather...
dot-tm_as_grangesRead 'regions' as an interval query against a GRanges source
dot-tm_complete_grFill in a GRanges that carries sequences but not their...
dot-tm_finishCompute Tm for one task's windows
dot-tm_matchResolve one identifier against a source's own names
dot-tm_modelApply the selected model to windows that already carry their...
dot-tm_offsetsWhere a source's records sit in the coordinate system the...
dot-tm_regionsResolve 'regions' against a source
dot-tm_runRun the tasks and reassemble one profile
dot-tm_sourceClassify the input and describe what it offers
dot-tm_task_bsgenomeOne task against a BSgenome
dot-tm_task_fastaOne task against a FASTA file
dot-tm_tasksCut the requested regions into tasks
ecoli_rep_hotspotsE. coli K-12 MG1655 replication-associated hotspot...
fa_to_genomic_rangesConvert FASTA file to GenomicRanges object
gc_contentCalculate G and C content of nucleotide sequences
generate_complementGenerate complementary sequence
integrate_grangesIntegrate a Tm GRanges with multi-omic feature ranges
make_genomiccoordGenerate sliding-window genomic coordinate strings for Tm...
plot_genome_trackPlot genome tracks in linear or circular layout
plot_tmCompare Tm distributions across groups
print.TmCalculatorPrints melting temperature from a 'TmCalculator' object
s2cconvert a string into a vector of characters
salt_correctCorrections of melting temperature with salt concentration
thermodynamic_gc_paramsThermodynamic parameters for GC-based Tm calculation methods
thermodynamic_nn_paramsThermodynamic Tables for Nucleic Acid Hybridization
tm_calculateCalculate melting temperature using multiple methods
TmCalculator-packageTmCalculator: Genome-Wide Nucleic Acid Melting Temperature...
tm_gcCalculate the melting temperature using empirical formulas...
tm_nnCalculate melting temperature using nearest neighbor...
tm_wallaceCalculate the melting temperature using the 'Wallace rule'
to_genomic_rangesConvert input sequences to a GRanges object (fast backend)
vec_to_genomic_rangesConvert sequence strings to GenomicRanges object
TmCalculator documentation built on Oct. 5, 2026, 5:08 p.m.