spots | R Documentation |
Find spots based on threshold and minimum total intensity
spots( img, mask, thresh.offset = 0.1, window = c(5, 5), min.sum.intensity = 0, zero = NA, max.spots = NULL, return = "intensity" )
img |
image array. |
mask |
mask array. |
thresh.offset |
threshold for minimum voxel intensity. |
window |
Half width and height of the moving rectangular window. |
min.sum.intensity |
threshold for minimum total spot intensity |
zero |
if NA, background is set to NA, if 0, background is set to 0. |
max.spots |
find max.spots spots with highest total intensity. |
return |
"mask" returns binarized mask, "intensity" returns intensity for spots, zero or NA otherwise "label" return labeled (numbered) spots. |
array
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