Neutrophils: Two-Photon Data: Neutrophils in an Infected Lung

In celltrackR: Motion Trajectory Analysis

Two-Photon Data: Neutrophils in an Infected Lung

Description

Labelled neutrophils were adoptively transfered and intravitally imaged (using two-photon microscopy) inside the lung of the recipient mouse. These cells display a fairly directed kind of motion, as they move towards infection foci.

Usage

data("Neutrophils")

Format

An S3 object of class "tracks"; a list with 10 elements. Each element name identifies a cell track. Each element is a matrix containing the following four columns.

t

the time (in seconds)

x

The X coordinate (in micrometers)

y

The Y coordinate (in micrometers)

z

The Z coordinate (in micrometers)

Source

Data were generated in 2012 in the Mark J. Miller Lab, Department of Medicine, Washington University in St Louis, USA.

References

Zinselmeyer BH, Dempster J, Wokosin DL, Cannon JJ, Pless R, Parker I and Miller MJ (2009), Two-photon microscopy and multi-dimensional analysis of cell dynamics. Methods in Enzymology, 461:349–78. doi:10.1016/S0076-6879(09)05416-0

Konjufca V and Miller MJ (2009), Imaging Listeria monocytogenes infection in vivo. Current Topics in Microbiology and Immunology, 334:199–226. doi:10.1007/978-3-540-93864-4_9

Kreisel D, Nava RG, Li W, Zinselmeyer BH, Wang B, Lai J, Pless R, Gelman AE, Krupnick AS, and Miller MJ (2010), In vivo two-photon imaging reveals monocyte-dependent neutrophil extravasation during pulmonary inflammation. PNAS, 107(42):18073–18078. doi:10.1073/pnas.1008737107

Examples

## load the tracks
data(Neutrophils)
## visualize the tracks (calls function plot.tracks)
plot(Neutrophils)

celltrackR documentation built on March 21, 2022, 5:06 p.m.