gl.filter.sexlinked: Filters loci that are sex linked

In dartR: Importing and Analysing 'SNP' and 'Silicodart' Data Generated by Genome-Wide Restriction Fragment Analysis

Filters loci that are sex linked

Description

Alleles unique to the Y or W chromosome and monomorphic on the X chromosomes will appear in the SNP dataset as genotypes that are heterozygotic in all individuals of the heterogametic sex and homozygous in all individuals of the homogametic sex. This function keeps or drops loci with alleles that behave in this way, as putative sex specific SNP markers.

Usage

gl.filter.sexlinked(
  x,
  sex = NULL,
  filter = NULL,
  read.depth = 0,
  t.het = 0.1,
  t.hom = 0.1,
  t.pres = 0.1,
  plot.out = TRUE,
  plot_theme = theme_dartR(),
  plot_colors = three_colors,
  verbose = NULL
)

Arguments

x	Name of the genlight object containing the SNP or presence/absence (SilicoDArT) data [required].
sex	Factor that defines the sex of individuals. See explanation in details [default NULL].
filter	Either 'keep' to keep sex linked markers only or 'drop' to drop sex linked markers [required].
read.depth	Additional filter option to keep only loci above a certain read.depth. Default to 0, which means read.depth is not taken into account [default 0].
t.het	Tolerance in the heterogametic sex, that is t.het=0.05 means that 5% of the heterogametic sex can be homozygous and still be regarded as consistent with a sex specific marker [default 0.1].
t.hom	Tolerance in the homogametic sex, that is t.hom=0.05 means that 5% of the homogametic sex can be heterozygous and still be regarded as consistent with a sex specific marker [default 0.1].
t.pres	Tolerance in presence, that is t.pres=0.05 means that a silicodart marker can be present in either of the sexes and still be regarded as a sex-linked marker [default 0.1].
plot.out	Creates a plot that shows the heterozygosity of males and females at each loci be regarded as consistent with a sex specific marker [default TRUE].
plot_theme	Theme for the plot. See Details for options [default theme_dartR()].
plot_colors	List of three color names for the not sex-linked loci, for the sex-linked loci and for the area in which sex-linked loci appear [default three_colors].
verbose	Verbosity: 0, silent or fatal errors; 1, begin and end; 2, progress log; 3, progress and results summary; 5, full report [default NULL, unless specified using gl.set.verbosity].

Details

Sex of the individuals for which sex is known with certainty can be provided via a factor (equal to the length of the number of individuals) or to be held in the variable x@other$ind.metrics$sex. Coding is: M for male, F for female, U or NA for unknown/missing. The script abbreviates the entries here to the first character. So, coding of 'Female' and 'Male' works as well. Character are also converted to upper cases.

' Function's output

This function creates also a plot that shows the heterozygosity of males and females at each loci for SNP data or percentage of present/absent in the case of SilicoDArT data.

Examples of other themes that can be used can be consulted in

• https://ggplot2.tidyverse.org/reference/ggtheme.html and

• https://yutannihilation.github.io/allYourFigureAreBelongToUs/ggthemes/

Value

The filtered genlight object (filter = 'keep': sex linked loci, filter='drop', everything except sex linked loci).

Author(s)

Arthur Georges, Bernd Gruber & Floriaan Devloo-Delva (Post to https://groups.google.com/d/forum/dartr)

See Also

Other filter functions: gl.filter.allna(), gl.filter.callrate(), gl.filter.heterozygosity(), gl.filter.hwe(), gl.filter.ld(), gl.filter.locmetric(), gl.filter.maf(), gl.filter.monomorphs(), gl.filter.overshoot(), gl.filter.parent.offspring(), gl.filter.pa(), gl.filter.rdepth(), gl.filter.reproducibility(), gl.filter.secondaries(), gl.filter.taglength()

Examples

  
out <- gl.filter.sexlinked(testset.gl, filter='drop')

out <- gl.filter.sexlinked(testset.gs, filter='drop')

dartR documentation built on June 8, 2023, 6:48 a.m.