UpsetjsPlot: UpsetjsPlot

In inDAGO: A GUI for Dual and Bulk RNA-Sequencing Analysis

UpsetjsPlot

Description

Create an interactive UpSet plot of overlapping DEGs using "UpsetJS".

Usage

UpsetjsPlot(
  WD_samples,
  Th_logFC,
  Th_Pvalue,
  collapseName,
  nintersects,
  st_significance
)

Arguments

WD_samples	Character. Directory containing DEG result CSV files.
Th_logFC	Numeric. Absolute log2 fold-change threshold to include a gene.
Th_Pvalue	Numeric. P-value threshold for significance (0 < Th_Pvalue <= 1).
collapseName	Logical. If TRUE, strip method/model prefixes from file names when labeling sets.
nintersects	Integer. Maximum number of intersections to display.
st_significance	Character. Which p-value to use: "adjustPvalue" (FDR or FWER) or "PValue".

Details

This function reads DEG CSV files from a directory, filters genes by log-FC and p-value thresholds (adjusted or raw), optionally simplifies file names, and visualizes the intersections of gene sets using the "UpsetJS" package.

1. Lists all CSV files in "WD_samples" and reads each into a data frame.

2. Checks for duplicate IDs and selects "ID", "logFC", and either "adjustPvalue" or "PValue".

3. Filters each set by "|logFC| >= Th_logFC" and p-value < "Th_Pvalue".

4. Renames each gene-ID list to the (optionally collapsed) file name.

5. Feeds the list of gene sets into "upsetjs::upsetjs()"

Value

An interactive "UpsetJS" object.

inDAGO documentation built on Aug. 8, 2025, 7:47 p.m.