Description Usage Arguments Value Author(s) Examples
Evaluation of an exact expression to obtain the percentage of all random nucleotide sequences of a given length (n) that can be sequenced with semiconductor sequencing given a certain number of flows (k).
1 | flowsRandom(k = 300, n = 150)
|
k |
Integer. Number of flows available during semiconductor sequencing. |
n |
Integer. Length of the random sequences to be sequenced. |
P |
Percentage of sequences that are completely covered |
Q |
Percentage of sequences that are not completely covered |
delta |
1 - (P + Q), should be a small number when calculation is accurate |
N |
Number of summands for calculation of P |
M |
Number of summands for calculation of Q |
summands |
N + M = total number of summands |
Michael Bockmayr and Jan Budczies
1 2 3 | ### To compute the percentage of covered sequences of length 150 with 300 flows run:
flowsRandom(300,150)
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