Sixteen mice from four strains were used; half were assigned to the treated condition and half to the control. The experiment was split into two batches, which were run two months apart.
A data frame with 16 rows and 4 variables:
Four strains of mice were used: NIH, BALB/c, A/J, and 129/Ola.
Presence or absence of diallyl sulphide.
The experiment was conducted in two batches.
Levels of glutathione-S-transferase (Gst).
The purpose of the experiment was to test if diallyl sulphide (DS) affects the activity of the liver enzyme Gst. Four strains of mice were used and the experiment was conducted in two batches, where the housing conditions differed between batches. Both batch and strain are considered blocking variables.
Festing MFW (2014). Randomized block experimental designs can increase the power and reproducibility of laboratory animal experiments. ILAR Journal 55(3):472-476.
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