Description Usage Arguments Value Examples
View source: R/normalization.R
Normalizes each replicate of an experiment based on a given spike-in protein standard (assumed to be present in equimolar amounts in each channel).
1 | normalize_to_std(expt, protein, model = T, plot = T)
|
expt |
an MSThermExperiment object |
protein |
ID of a protein to normalize against |
model |
whether to fit scale factors to model |
plot |
(T/f) whether to display a summary plot |
An MsThermExperiment object with normalized data slots
1 2 3 4 5 | control <- system.file("extdata", "demo_project/control.tsv", package="mstherm")
annots <- system.file("extdata", "demo_project/annots.tsv", package="mstherm")
expt <- MSThermExperiment(control, annotations=annots)
expt <- normalize_to_std(expt, "cRAP_ALBU_BOVIN", plot=FALSE)
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