normalize_RFU: Normalize Fluorescence

In quicR: RT-QuIC Data Formatting and Analysis

Normalize Fluorescence

Description

Normalizes the real-time RT-QuIC data against the background fluorescence of a defined cycle. All cycles are divided by the fluorescent value of the defined cycle.

Usage

normalize_RFU(data, bg_cycle = 4, transposed = FALSE)

Arguments

data	A dataframe generated from get_real.
bg_cycle	The cycle used for background fluorescence
transposed	Logical, TRUE if cycle values are shown as column names.

Value

A dataframe containing real-time normalized fluorescence values.

Examples

# This test takes >5 sec

file <- system.file(
  "extdata/input_files",
  file = "test2.xlsx",
  package = "quicR"
)
df_ <- get_real(file)[[1]]

# Export the tables in the first sheet of the file.
dic <- quicR::organize_tables(file)

# Normalize the raw data against the background reading.
normalize_RFU(df_)

quicR documentation built on April 3, 2025, 11:20 p.m.