bibi: Kinetic Mechanisms and Parameters for Bi-Bi Reactions

In renz: R-Enzymology

Kinetic Mechanisms and Parameters for Bi-Bi Reactions

Description

Discriminates between sequential and ping-pong mechanisms and estimates the kinetic parameters.

Usage

bibi(data, unit_a = "mM", unit_b = "mM", unit_v = "ua", vice_versa = FALSE)

Arguments

data	either a dataframe or the path to a text file containing the data (see details).
unit_a	concentration unit for substrate A.
unit_b	concentration unit for substrate B.
unit_v	velocity unit.
vice_versa	logical. When FALSE the variable substrate is A. If TRUE, then the variable substrate is B.

Details

Either the txt file or the dataframe containing the data must conform to the following format: a table with three columns and as many rows as conditions were assessed. The first and second columns are named 'a' and 'b' and they give the concentrations for substrate A and B, respectively. The third column, named 'rate', provides the assessed rates.

Value

A list with three elements: (i) a character vector giving the kinetic parameters Vmax, KiA, Km_A and Km_B values; (ii) a numeric vector giving the apparent inverse of Vmax for each concentration of substrate B (intercepts of primary representation); and (iii) a numeric vector giving the apparent specificity constant for each concentration of substrate B (slopes from primary representations).

Examples

bibi(data = hk)

renz documentation built on Nov. 27, 2023, 5:11 p.m.