lb: Lineweaver-Burk Transformation

In renz: R-Enzymology

Lineweaver-Burk Transformation

Description

Obtains Km and Vm using double reciprocal transformation

Usage

lb(data, unit_S = 'mM', unit_v = 'au', weighting = FALSE, plot = TRUE)

Arguments

data	a dataframe where the first column is the independent variable, [S], and the remaining columns (as many as experiment replicates) correspond to the dependent variable, v.
unit_S	concentration unit.
unit_v	time unit.
weighting	logical. When TRUE the weight v^4 is employed.
plot	logical. If TRUE the data and fitted line are plotted.

Value

A double reciprocal plot and the Km and Vm computed using averaged 1/v (when more than one replicate is provided). In addition, this function returns a list of five elements. The first and second ones are vectors with the Km and Vm, respectively, computed individually for each replicate. The third one provides the R-squared values of the fits. The fourth element of the list gives the fitted Km and Vm. The last element of the list is a dataframe with the values of the transformed variables.

References

J. Am. Chem. Soc.1934, 56, 3,658-666 (doi.org/10.1021/ja01318a036)

See Also

hw(), eh(), ecb()

Examples

lb(ONPG[, c(1,2)], weighting = TRUE)

renz documentation built on Nov. 27, 2023, 5:11 p.m.