ecb: Eisenthal & Cornish-Bowden

In renz: R-Enzymology

Eisenthal & Cornish-Bowden

Description

Obtains Km and Vm using the Eisenthal & Cornish-Bowden method.

Usage

ecb(data, unit_S = 'mM', unit_v = 'au', plot = TRUE)

Arguments

data	a dataframe where the first column is the independent variable, [S], and the remaining columns (as many as experiment replicates) correspond to the dependent variable, v.
unit_S	concentration unit.
unit_v	time unit.
plot	logical. If TRUE data are plotted.

Details

For each experimental replicate the observations (S, v) are plotted as lines in the Km-Vm parameter space, instead of points in observation space. Afterwards, the lines tend to intersect at a common point, whose coordinates provide the kinetic parameters. Nevertheless, since the observations are subject to error, there is no unique intersection point for all the lines. In this case, the method computes all the pair-wise intersections. Then, the median value from each series is taken to be the best estimate of Km and Vm. This procedure is repeated as many times as replicates and finally the mean and sd is returned.

Value

Returns a list with the estimated values of Km and Vm.

References

Biochem.J.(1974) 139:715-720 (10.1042/bj1390715)

See Also

lb(), hw(), eh()

Examples

ecb(ONPG[, c(1,2)])

renz documentation built on Nov. 27, 2023, 5:11 p.m.