# closed: Closed respirometry In respirometry: Tools for Conducting and Analyzing Respirometry Experiments

## Description

Returns the unknown parameter given 3 of 4 parameters to calculate respiration rate in a closed respirometer. This is useful both for basic closed respirometry setups, and also for the closed measurement phase of intermittent respirometry.

## Usage

 ```1 2``` ```closed(MO2, delta_pO2, duration, vol, temp = 25, sal = 35, atm_pres = 1013.25) ```

## Arguments

 `MO2` whole-animal oxygen consumption rate (umol O2 / hour). `delta_pO2` desired change in pO2 (% air saturation). `duration` desired duration to reach `delta_pO2` (minutes). `vol` volume of the respirometer (liter). `temp` temperature (°C). Default is 25 °C. `sal` salinity (psu). Default is 35 psu. `atm_pres` atmospheric pressure (mbar). Default is 1013.25 mbar.

## Note

If there are more than two O2 observations, consider using `calc_MO2`.

## Author(s)

Matthew A. Birk, [email protected]

`flush_water`, `calc_MO2`
 ``` 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17``` ```# I've read in the literature that my animal has an SMR of 200 umol/h. How large of a # respirometer do I want if I want it to breathe down to 80% air saturation in 30 minutes? closed(MO2 = 200, delta_pO2 = 100 - 80, duration = 30) # returns respirometer volume # I've read in the literature that my animal has an SMR of 1000 umol/h. How long will it take to # breathe down a 50 L respirometer by 10% air saturation? closed(MO2 = 1000, delta_pO2 = 10, vol = 50) # returns the duration to breathe down the O2 # How does animal size affect how long my measurement periods last? mass_range <- seq(100, 400, 50) dur_range <- (closed(MO2 = scale_MO2(mass_1 = 100, MO2_1 = 400, mass_2 = mass_range), delta_pO2 = 20, vol = 10)) plot(mass_range, dur_range, type = 'b') # What is the MO2 if O2 drops 0.44 mg/l in 33 minutes when the respirometer volume is 30 L? closed(delta_pO2 = conv_o2(o2 = 0.44, from = 'mg_per_l', to = 'percent_a.s.'), duration = 33, vol = 30) ```