three_mode: Create Three Mode PCA and PCoA plots

In tidyMicro: A Pipeline for Microbiome Analysis and Visualization

Description

Three Mode Principal Components, an ordination method that can take into account repeated measure of subjects. These methods have also been extended to other common ecological distance metrics for Three Mode Principal Coordinate Analysis

Usage

three_mode(
  micro_set,
  table,
  group,
  time_var,
  subject,
  y = clr,
  plot_scores = F,
  main = NULL,
  subtitle = NULL,
  legend_title = NULL,
  scalewt = TRUE
)

Arguments

micro_set	A tidy_micro data set
table	OTU table of interest
group	A categorical variable to color by
time_var	The time point variable column name in your tidi_MIBI set
subject	The subject variable column name in your tidi_MIBI set
y	Value to calculate principle components or coordinates on. Default is centered log ratio (recommended)
plot_scores	Plot the scores instead of the principle components
main	Plot title
subtitle	Plot subtitle
legend_title	Plot legend title
scalewt	Logical; center and scale OTU table, recommended

Details

Requires that you have columns for subject name and time point. Data must be complete across time points. The function will filter out inconsistent subjects

If n_compA, n_compB, and n_compC aren't specified they will default to the number of complete subjects, the number of taxa, and the number of time points, respectively. This slows down performance slightly, but will not change the results.

Value

A ggplot you can add geoms to if you'd like

Author(s)

Charlie Carpenter, Kayla Williamson

Examples

data(bpd_phy); data(bpd_cla); data(bpd_ord); data(bpd_fam); data(bpd_clin)
otu_tabs = list(Phylum = bpd_phy, Class = bpd_cla,
Order = bpd_ord, Family = bpd_fam)

set <- tidy_micro(otu_tabs = otu_tabs, clinical = bpd_clin)

set %>% three_mode(table = "Family", group = bpd1, time_var = day, subject = study_id)

tidyMicro documentation built on Jan. 13, 2021, 6:18 a.m.