dot-callP3NreadOrg: call primer3 for a given set of DNAstringSet object

In Acribbs/scAmpDesign:

Description

call primer3 for a given set of DNAstringSet object

Usage

.callP3NreadOrg(
  seq,
  size_range = "200-700",
  Tm = c(58, 60, 70),
  name = "Hodor",
  primer3 = NULL,
  thermo.param = NULL,
  settings = NULL,
  excluded_regions = NULL
)

Arguments

seq	DNAstring object, one DNA string for the given amplicon
size_range	default: '151-500'
Tm	melting temprature parameters default:c(55,57,58)
name	name of the amplicon in chr_start_end format
primer3	primer3 location
settings	text file for parameters
therme.param	thermodynamic parameters folder

Author(s)

Altuna Akalin modified Arnaud Krebs' original function

Acribbs/scAmpDesign documentation built on May 26, 2020, 9:56 p.m.