make_bigwigs: Generate Bigwigs

Description Usage Arguments

View source: R/bigwigs.R

Description

Generate Bigwigs

Usage

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make_bigwigs(
  zent_obj,
  outdir = getwd(),
  bin_size = 10,
  normalize_using = NA,
  genome_size = NA,
  skip_non_covered = TRUE,
  min_fragment = NA,
  max_fragment = NA,
  extend_reads = 200,
  scale_factors = NA,
  split_strands = FALSE,
  library_type = NA,
  temp_dir = "./temp"
)

Arguments

zent_obj

ZentTools object.

outdir

Output directory.

bin_size

Bin size for coverage summary.

normalize_using

Either 'CPM' or 'RPGC'.

genome_size

Effective genome size.

skip_non_covered

Should regions without coverage be skipped.

min_fragment

Minimum fragment length.

max_fragment

Maximum fragment length.

extend_reads

Distance to extend single-end reads. Set to NA to not extend reads.

scale_factors

Takes a named vector, with the name being the sample name, and the value being the scale factor. If set will override 'normalzie_using' option.

split_strands

For RNA-seq, whether to split the strands into positive and minus strand files.

library_type

If split_strands is TRUE, specify library chemistry as either 'dUTP' or 'ligation'.

temp_dir

Temporary directory to write files to.


Bankso/ZentTools_too documentation built on Jan. 18, 2021, 5:36 p.m.