xy2indices: Functions to convert indices to x/y (and reverse)

In Bioconductor/affy: Methods for Affymetrix Oligonucleotide Arrays

Functions to convert indices to x/y (and reverse)

Description

Functions to convert indices to x/y (and reverse)

Usage

xy2indices(x, y, nc = NULL, cel = NULL, abatch = NULL, cdf = NULL, xy.offset = NULL)
indices2xy(i, nc = NULL, cel = NULL, abatch = NULL, cdf = NULL, xy.offset = NULL)

Arguments

x	A numeric vector of X (column) position(s) for the probes.
y	A numeric vector of Y (row) position(s) for the probes.
i	A numeric vector of indices in the AffyBatch for the probes.
nc	total number of columns on the chip. It is usually better to specify either the cdf or abatch arguments rather than the number of columns.
cel	a corresponding object of class Cel. This has been deprecated. Use abatch or cdf instead.
abatch	a corresponding object of class AffyBatch.
cdf	character - the name of the corresponding cdf package.
xy.offset	an eventual offset for the XY coordinates. See Details.

Details

The Affymetrix scanner reads data from a GeneChip by row, and exports those data to a CEL file. When we read in the CEL file data to an AffyBatch object, we store data for each GeneChip as a single column in a matrix of probe-wise intensity values.

The CDF files that Affymetrix make available for various GeneChips map individual probes to probesets based on their (x,y) coordinates on the GeneChip. Note that these coordinates are zero-based, and (x,y) is the same as (column, row). In other words, the x coordinate indicates the horizontal location of the probe, and the y coordinate indicates the vertical location of the probe. By convention, (0,0) is the coordinate location for the top left position, and (ncol-1, nrow-1) is the coordinate location of the lower right position.

For most users, the mapping of probes to probeset is handled internally by various functions (rma, espresso, etc), and in general usage it is never necessary for a user to convert probe index position in an AffyBatch to the corresponding (x,y) coordinates on the GeneChip. These functions are only useful for those who wish to know more about the internal workings of the Affymetrix GeneChip.

The parameter xy.offset is there for compatibility. For historical reasons, the xy-coordinates for the features on Affymetrix GeneChips were decided to start at 1 (one) rather than 0 (zero). One can set the offset to 1 or to 0. Unless the you \_really\_ know what you are doing, it is advisable to let it at the default value NULL. This way the package-wide option xy.offset is always used.

Value

A vector of indices or a two-columns matrix of Xs and Ys.

Warning

Even if one really knows what is going on, playing with the parameter xy.offset could be risky. Changing the package-wide option xy.offset appears much more sane.

Author(s)

L.

See Also

indexProbes

Examples

if (require(affydata)) {
  data(Dilution)
  pm.i <- indexProbes(Dilution, which="pm", genenames="AFFX-BioC-5_at")[[1]]
  mm.i <- indexProbes(Dilution, which="mm", genenames="AFFX-BioC-5_at")[[1]]

  pm.i.xy <- indices2xy(pm.i, abatch = Dilution)
  mm.i.xy <- indices2xy(mm.i, abatch = Dilution)

  ## and back to indices
  i.pm <- xy2indices(pm.i.xy[,1], pm.i.xy[,2], cdf = "hgu95av2cdf")
  i.mm <- xy2indices(mm.i.xy[,1], mm.i.xy[,2], cdf = "hgu95av2cdf")

  identical(pm.i, as.integer(i.pm))
  identical(mm.i, as.integer(i.mm))

  image(Dilution[1], transfo=log2)
  ## plot the pm in red
  plotLocation(pm.i.xy, col="red")
  plotLocation(mm.i.xy, col="blue")
}

Bioconductor/affy documentation built on July 21, 2023, 5:23 p.m.