gwpcr.sd: PCR Product Distribution Standard Deviation

In Cibiv/gwpcR: Amplification+Sequencing Model Based on a Galton-Watson Branching Process and Poissonian Sampling

Description

For efficiency E and initial number of molecules m, the PCR product distribution has variance (not standard deviation!) (1+E) / ((1-E) * m).

Function gwpcr.sd uses this to compute the standard deviation (i.e. the square of the above) for a given efficiency and initial copy number, and gwpcr.sd.inv does the reverse and computes the efficiency given standard deviation and copy number.

Usage

gwpcr.sd(efficiency, molecules = 1)

gwpcr.sd.inv(sd, molecules = 1)

Arguments

efficiency	efficiency of amplification
molecules	initial copy number
sd	Standard deviation of the PCR product distribution

See Also

gwpcr

Cibiv/gwpcR documentation built on Aug. 31, 2021, 1:20 p.m.