A problem when recording 3D fluorescent microscopy images is how to properly present these results in 2D. Maximum intensity projections are a popular method to determine the focal plane of each pixel in the image. The problem with this approach, however, is that out-of-focus elements will still be visible, making edges and fine structures difficult to detect. This package aims to resolve this problem by using the contrast around a given pixel to determine the focal plane, allowing for a much cleaner structure detection than would be otherwise possible. For convenience, this package also contains functions to perform various other types of projections, including a maximum intensity projection.
|Author||Jan Sauer, Bernd Fischer|
|Bioconductor views||CellBasedAssays Preprocessing Software Visualization|
|Maintainer||Jan Sauer <email@example.com>|
|Package repository||View on GitHub|
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