integrateF_Fo: A function used to quantify the response in the region of...

In FafferMcgee/calcium-analysis:

Description

It normalizes the intensity data during stimulus window by calculating an average Fo before stimulus, and then dviding the fluorescence values in this window by the background Fo.

Usage

integrateF_Fo(data, conditions, averageWindow)

Arguments

data	= Dataframe containing fluorescence intensity data
conditions	= vectory of integers indicating when
averageWindow	= the window to use prior to stimulus for calculating Fo i = odd index of conditions (corresponds of start of new condition) j = index of response

Details

Normalizing first by an averaged background (Fo) right before stimulus corrects for photobleaching/drift.

FafferMcgee/calcium-analysis documentation built on Sept. 3, 2020, 12:07 a.m.