quantF_Fo: A function used to quantify the response in the region of...

Description Usage Details

View source: R/quantF_Fo.R

Description

It normalizes the intensity data during stimulus window by calculating an average Fo before stimulus, and then dviding the fluorescence values in this window by the background Fo.

Usage

1
quantF_Fo(data, conditions, averageWindow)

Details

Normalizing first by an averaged background (Fo) right before stimulus corrects for photobleaching/drift.

Inputs: Data = Dataframe containing fluorescence intensity data Conditions = vectory of integers indicating when averageWindow = the window to use prior to stimulus for calculating Fo

i = odd index of conditions (corresponds of start of new condition) j = index of response


FafferMcgee/calcium-analysis documentation built on Sept. 3, 2020, 12:07 a.m.