SCREEN(Single-cell CRISPR scREen data analyses and pErturbation modeliNg) is a pipeline to visualize the quality of single-cell CRISPR screens RNA-seq/ATAC-seq datasets. SCREEN has integrated three R functions: scMAGeCK_lr, Mixscape and plot function of cicero. These functions are used to estimate the regulatory score between perturbations and genes, estimate the perturbation efficiency and visualize enhancer regulatory potential, respectively.
We are hosting SCREEN documentation, instruction and tutorials at SCREEN Website.
R >= 4.0.3
Seurat >= 4.0.3
scMAGeCK
snakemake
mixtools
$ R
> library(devtools)
> install_github("HailinWei98/SCREEN")
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