consolidatePairs: Consolidate results for interactions

In LTLA/diffHic: Differential Analysis of Hi-C Data

Consolidate results for interactions

Description

Consolidate differential testing results for interactions from separate analyses.

Usage

consolidatePairs(indices, result.list, equiweight=TRUE, combine.args=list())

Arguments

indices	a list of index vectors, specifying the cluster ID to which each interaction belongs
result.list	a list of data frames containing the DI test results for each interaction
equiweight	a logical scalar indicating whether equal weighting from each bin size should be enforced
combine.args	a list of parameters to pass to combineTests

Details

Interactions from different analyses can be aggregated together using boxPairs or clusterPairs. For example, test results can be consolidated for bin pairs of differing sizes. This usually produces a indices vector that can be used as an input here. Briefly, each vector in indices should correspond to one analysis, and each entry of that vector should correspond to an analyzed interaction. The vector itself holds cluster IDs, such that interactions within/between analyses with the same ID belong in the same cluster.

For all bin pairs in a cluster, the associated p-values are combined in combineTests using a weighted version of Simes' method. This yields a single combined p-value, representing the evidence against the global null. When equiweight=TRUE, the weight of a p-value of each bin pair is inversely proportional to the number of bin pairs of the same size in that parent bin pair. This ensures that the results are not dominated by numerous smaller bin pairs.

Value

A data frame is returned containing the combined DB results for each cluster.

Author(s)

Aaron Lun

See Also

combineTests, boxPairs, clusterPairs

Examples

# Setting up the objects.
a <- 10
b <- 20
cuts <- GRanges(rep(c("chrA", "chrB"), c(a, b)), IRanges(c(1:a, 1:b), c(1:a, 1:b)))
param <- pairParam(cuts)

all.combos <- combn(length(cuts), 2) # Bin size of 1.
y <- InteractionSet(matrix(0, ncol(all.combos), 1), 
    GInteractions(anchor1=all.combos[2,], anchor2=all.combos[1,], regions=cuts, mode="reverse"),
    colData=DataFrame(lib.size=1000), metadata=List(param=param, width=1))

a5 <- a/5
b5 <- b/5
all.combos2 <- combn(length(cuts)/5, 2) # Bin size of 5.
y2 <- InteractionSet(matrix(0, ncol(all.combos2), 1), 
    GInteractions(anchor1=all.combos2[2,], anchor2=all.combos2[1,], 
	    regions=GRanges(rep(c("chrA", "chrB"), c(a5, b5)), 
		    IRanges(c((1:a5-1)*5+1, (1:b5-1)*5+1), c(1:a5*5, 1:b5*5))), mode="reverse"),
     colData=DataFrame(lib.size=1000), metadata=List(param=param, width=5))

result1 <- data.frame(logFC=rnorm(nrow(y)), PValue=runif(nrow(y)), logCPM=0)
result2 <- data.frame(logFC=rnorm(nrow(y2)), PValue=runif(nrow(y2)), logCPM=0)

# Consolidating.
boxed <- boxPairs(y, y2)
out <- consolidatePairs(boxed$indices, list(result1, result2))
head(out)
out <- consolidatePairs(boxed$indices, list(result1, result2), equiweight=FALSE)
head(out)

# Repeating with three sizes.
a10 <- a/10
b10 <- b/10
all.combos3 <- combn(length(cuts)/10, 2) # Bin size of 10.
y3 <- InteractionSet(matrix(0, ncol(all.combos3), 1), 
    GInteractions(anchor1=all.combos3[2,], anchor2=all.combos3[1,], 
    	regions=GRanges(rep(c("chrA", "chrB"), c(a10, b10)), 
	    	IRanges(c((1:a10-1)*10+1, (1:b10-1)*10+1), c(1:a10*10, 1:b10*10))),
        mode="reverse"),
     colData=DataFrame(lib.size=1000), metadata=List(param=param, width=10))
result3 <- data.frame(logFC=rnorm(nrow(y3)), PValue=runif(nrow(y3)), logCPM=0)

boxed <- boxPairs(y, y2, y3)
out <- consolidatePairs(boxed$indices, list(result1, result2, result3))
head(out)

LTLA/diffHic documentation built on Dec. 20, 2024, 7:06 p.m.