filterDiag: Filtering of diagonal bin pairs

In LTLA/diffHic: Differential Analysis of Hi-C Data

Filtering of diagonal bin pairs

Description

Filtering to remove bin pairs on or near the diagonal of the interaction space.

Usage

filterDiag(data, by.dist=0, by.diag=0L, dist, ...)

Arguments

data	an InteractionSet object produced by squareCounts
by.dist	a numeric scalar indicating the base-pair distance threshold below which bins are considered local
by.diag	an integer scalar indicating the bin distance threshold below which bins are considered local
dist	a optional numeric vector containing pre-computed distances
...	other arguments to pass to pairdist, if dist is not specified

Details

Pairs of the same bin will lie on the diagonal of the interaction space. Counts for these pairs can be affected by local artifacts (e.g., self-circles, dangling ends) that may not have been completely removed during earlier quality control steps. These pairs are also less interesting, as they capture highly local structure that may be the result of non-specific compaction. In many cases, these bin pairs are either removed or, at least, normalized separately within the analysis.

This function provides a convenience wrapper in order to separate diagonal bin pairs from those in the rest of the interaction space. Users can also consider near-diagonal bin pairs, which are defined as pairs of local bins on the linear genome. Specifically, bins are treated as local if they separated by less than by.dist in terms of base pairs, or by less than by.diag in terms of bins. These can be separated with the diagonal bin pairs if they are subject to the same issues described above.

Note that if by.dist is specified, it should be set to a value greater than 1.5 times the average bin size. Otherwise, the distance between the midpoints of adjacent bins will always be larger than by.dist, such that no near-diagonal bin pairs are removed.

Users can expedite processing by supplying a pre-computed vector of distances in dist. This vector may already be available if it was generated elsewhere in the pipeline. However, the supplied vector should have the same number of entries as that in data.

Value

A logical vector indicating whether each bin pair in data is a non-diagonal (or non-near-diagonal) element.

Author(s)

Aaron Lun

See Also

pairdist

Examples

hic.file <- system.file("exdata", "hic_sort.bam", package="diffHic")
cuts <- readRDS(system.file("exdata", "cuts.rds", package="diffHic"))
param <- pairParam(fragments=cuts)

# Setting up the parameters
fout <- tempfile(fileext=".h5")
invisible(preparePairs(hic.file, param, file=fout))

# Collating to count combinations.
y <- squareCounts(fout, param, width=50, filter=1)

summary(filterDiag(y))
summary(filterDiag(y, by.dist=100))
summary(filterDiag(y, by.diag=1))
summary(filterDiag(y, dist=pairdist(y)))

LTLA/diffHic documentation built on Dec. 20, 2024, 7:06 p.m.