In PeterDeWeirdt/intactr: Detect Interactions from CRISPR Combinatorial Screens

knitr::opts_chunk$set(
  collapse = TRUE,
  comment = "#>",
  fig.width = 8,
  fig.height = 5
)

library(intactr)

Calculate genetic interaction scores for a programmed pair library screened with Cas12a

The data: 12 pre-selected gene pairs Max 20 guides per gene 25 olfactory receptor controls Dual orientation Three direct repeats columns gene1, guide1 (guide1:DR), guide2 (guide2:DR), control1, control2

knitr::kable(head(prog_pairs))

combo_scores <- calculate_anchor_residuals(prog_pairs, 60, fit_controls = T)

PeterDeWeirdt/intactr documentation built on June 8, 2020, 1:52 p.m.