groupBy: group the rows and ranges of the profileplyr object

In RockefellerUniversity/profileplyr: Visualization and annotation of read signal over genomic ranges with profileplyr

group the rows and ranges of the profileplyr object

Description

group the rows and ranges of the profileplyr object

Usage

groupBy(
  object = "profileplyr",
  group = "ANY",
  GRanges_names = "character",
  levels = "ANY",
  include_nonoverlapping = "logical",
  separateDuplicated = "logical",
  inherit_groups = "logical"
)

## S4 method for signature 'profileplyr'
groupBy(
  object = "profileplyr",
  group = "ANY",
  GRanges_names = NULL,
  levels = NULL,
  include_nonoverlapping = FALSE,
  separateDuplicated = TRUE,
  inherit_groups = FALSE
)

Arguments

object	A profileplyr object
group	How the ranges will be grouped. If this is a character string, then it must match a column name of the range metadata, and this column will be used for grouping of any exported deepTools matrix. If this is a GRanges, or GRangesList, then the ranges will be subset based on overlap with these GRanges. If this is a list, each element should contain ether 1) a character vector of genes, and ranges will be subset based on overlap with these genes, as determined by the annotations made by annotateRanges() or annotateRanges_great() functions, or 2) a data frame with the gene symbols as the rownames. Any additional columns of this dataframe will be added to the range metadata.
GRanges_names	The names of the GRanges that were used for the "GRanges" argument. This will be used to label these groups in the construction of the resulting profileplyr object.
levels	This will set the levels of the grouping column set by 'rowGroupsInUse' (if the grouping column is not a factor, it will be converted to one). If levels are not provided, they will remain unchanged if the grouping column was already a factor, or will use default leveling (e.g. alphabetical) if grouping column is not already a factor variable.
include_nonoverlapping	A logical argument, if FALSE (default) the regions from the original deepTools matrix that do not overlap with the user defined regions will be left out of the returned profileplyr object.
separateDuplicated	A logical argument, if TRUE (default) then regions that overlap multiple inputs to 'GRanges' argument will be separated and made into their own group. All possible combinations of region overlaps will be tested, so it is not recommended to have more than 3 groups if this option is TRUE. If FALSE, then regions that overlap each individual 'GRanges' input will be in the output, and if one region overlaps multiple 'GRanges' inputs, then it will be duplicated in the output and will show up in the section for each group.
inherit_groups	A logical whether that groups the exist in the profileplyr object in the 'object' argument should be included in the default grouping scheme for the output object of this function. The default is TRUE. If false, only the GRanges or gene list overlap annotation will be used for heatmap grouping.

Details

Takes a SE object and groups rows

Value

A profileplyr object

Methods (by class)

• groupBy(profileplyr): group the rows and ranges of the profileplyr object

Examples

# group by gene list or list of data frames with genes as rownames
## not shown here but see vignette for grouping by gene lists

# group by GRanges

example <- system.file("extdata", "example_deepTools_MAT", package = "profileplyr") 
object <- import_deepToolsMat(example)
data("K27ac_GRlist_hind_liver_top5000") # load pre-made GRanges
K27ac_groupByGR <- groupBy(object, group = K27ac_GRlist_hind_liver_top5000)

# switch rowGroupsInUse

switchGroup <- groupBy(K27ac_groupByGR, group = "GR_overlap_names")
params(switchGroup)$rowGroupsInUse

RockefellerUniversity/profileplyr documentation built on Jan. 28, 2023, 10:09 a.m.