calc.po.dtm: Calculate adjustment to melting temperature for...

In Santaris/affinity: Functions to do thermodynamics on oligonucleotides

Description

See calculate.affinity for background.

Usage

calc.po.dtm(oligos, conc.total = 1e-05, parallel=F, cores=NA)

Arguments

oligos	A character vector of oligonucleotide sequences in camel-case (uppercase: LNA, lowercase: DNA) in the 5' to 3' direction.
conc.total	A numeric, the total concentration (in moles per liter, M) of oligonucleotide and RNA target (assumed to be present 1:1, so that the concentration of each equals half the total concentration). This is used for calculating melting temperature (tm).
parallel	logical(T/F), should the computation be parallelized.
cores	integer, the number of cores used for parallel computation. If NA, use the maximal number of cores. This parameter is only relevant if parallel=T.

Details

For the current dtm calculation, all oligonucloetides are treated as DNA only.

Value

A numeric vector with the dtm's.

Author(s)

Peter Hagedorn and Morten Lindow

See Also

calculate.affinity

Examples

calc.po.dtm(rep("AAAgtacaaTTG",10))

Santaris/affinity documentation built on May 9, 2019, 12:43 p.m.