demuxBarplot: Barplot of Illumina demultiplexing statistics.

In ThomasCarroll/basecallQC: Working with Illumina Basecalling and Demultiplexing input and output files

Description

Produces a barplot of demultiplexing statistics of reads with perfect/mismatched barcode.

Usage

## S4 method for signature 'baseCallQC'
demuxBarplot(object,groupBy)

## S4 method for signature 'basecallQC'
demuxBarplot(object = "basecallQC",
  groupBy = c("Lane"))

## S4 method for signature 'list'
demuxBarplot(object = "basecallQC", groupBy = c("Lane"))

Arguments

object	A basecallQC object or list from call to demultiplexMetrics()
groupBy	Character vector of how data is grouped for plotting. Should be either "Project","Sample" or "Lane".

Value

A ggplot2 object.

Author(s)

Thomas Carroll and Marian Dore

Examples

fileLocations <- system.file("extdata",package="basecallQC")
runXML <- dir(fileLocations,pattern="runParameters.xml",full.names=TRUE)
config <- dir(fileLocations,pattern="config.ini",full.names=TRUE)
sampleSheet <- dir(fileLocations,pattern="*\\.csv",full.names=TRUE)
outDir <- file.path(fileLocations,"Runs/161105_D00467_0205_AC9L0AANXX/C9L0AANXX/")
bcl2fastqparams <- BCL2FastQparams(runXML,config,runDir=getwd(),outDir,verbose=FALSE)
bclQC <- basecallQC(bcl2fastqparams,RunMetaData=NULL,sampleSheet)
plot <- demuxBarplot(bclQC)

ThomasCarroll/basecallQC documentation built on May 9, 2019, 4:45 p.m.