Description Usage Arguments Value
Title
1 2 3 4 5 6 7 8 9 10 11 |
pr_data |
a long data.frame containing you plate reader data |
blank_well |
the well coordinates of a media blank |
neg_well |
the well coordinates of a non-fluorescent control |
OD_name |
the column name for the optical density data |
flu_names |
the column names for the fluorscence data |
to_MEFL |
a Boolean to determine whether to attempt to convert OD and GFP reading to calibrated units |
GFP_gain |
if to_MEFL=T, the gain value at which GFP was recorded |
lid_type |
if to_MEFL=T, the sealing mechanism used on the microtitre-plate. See the conversion_factors_csv for available options |
conversion_factors_csv |
if to_MEFL=T, path of the csv file containing conversion factors from plate reader calibration |
a data.frame with columns for raw plate reader data, normalised data and, if to_MEFL=T, calibrated OD and GFP data
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