prNorm: Title

In ajfedorec/prNorm: Perform normalisation of plate reader data

Description

Title

Usage

prNorm(
  pr_data,
  blank_well = "A1",
  neg_well = "A2",
  OD_name = "OD",
  flu_names = c("GFP"),
  to_MEFL = F,
  GFP_gain = 0,
  lid_type = "nolid",
  conversion_factors_csv = NA
)

Arguments

pr_data	a long data.frame containing you plate reader data
blank_well	the well coordinates of a media blank
neg_well	the well coordinates of a non-fluorescent control
OD_name	the column name for the optical density data
flu_names	the column names for the fluorscence data
to_MEFL	a Boolean to determine whether to attempt to convert OD and GFP reading to calibrated units
GFP_gain	if to_MEFL=T, the gain value at which GFP was recorded
lid_type	if to_MEFL=T, the sealing mechanism used on the microtitre-plate. See the conversion_factors_csv for available options
conversion_factors_csv	if to_MEFL=T, path of the csv file containing conversion factors from plate reader calibration

Value

a data.frame with columns for raw plate reader data, normalised data and, if to_MEFL=T, calibrated OD and GFP data

ajfedorec/prNorm documentation built on Dec. 6, 2020, 9:13 a.m.