prNorm: Title

Description Usage Arguments Value

View source: R/prNorm.R

Description

Title

Usage

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prNorm(
  pr_data,
  blank_well = "A1",
  neg_well = "A2",
  OD_name = "OD",
  flu_names = c("GFP"),
  to_MEFL = F,
  GFP_gain = 0,
  lid_type = "nolid",
  conversion_factors_csv = NA
)

Arguments

pr_data

a long data.frame containing you plate reader data

blank_well

the well coordinates of a media blank

neg_well

the well coordinates of a non-fluorescent control

OD_name

the column name for the optical density data

flu_names

the column names for the fluorscence data

to_MEFL

a Boolean to determine whether to attempt to convert OD and GFP reading to calibrated units

GFP_gain

if to_MEFL=T, the gain value at which GFP was recorded

lid_type

if to_MEFL=T, the sealing mechanism used on the microtitre-plate. See the conversion_factors_csv for available options

conversion_factors_csv

if to_MEFL=T, path of the csv file containing conversion factors from plate reader calibration

Value

a data.frame with columns for raw plate reader data, normalised data and, if to_MEFL=T, calibrated OD and GFP data


ajfedorec/prNorm documentation built on Dec. 6, 2020, 9:13 a.m.