NcdfGenotypeReader-class: Class NcdfGenotypeReader

In amstilp/GWASTools: Tools for Genome Wide Association Studies

Description

The NcdfGenotypeReader class is an extension of the NcdfReader class specific to reading genotype data stored in NetCDF files.

Extends

NcdfReader

Constructor

NcdfGenotypeReader(filename):

filename must be the path to a NetCDF file. The NetCDF file must contain the following variables:

• 'snp': a coordinate variable with a unique integer vector of snp ids

• 'chromosome': integer chromosome codes of dimension 'snp'

• 'position': integer position values of dimension 'snp'

• 'sampleID': a unique integer vector of scan ids with dimension 'sample'

• 'genotype': a matrix of bytes with dimensions ('snp','sample'). The byte values must be the number of A alleles : 2=AA, 1=AB, 0=BB.

Default values for chromosome codes are 1-22=autosome, 23=X, 24=XY, 25=Y, 26=M. The defaults may be changed with the arguments autosomeCode, XchromCode, XYchromCode, YchromCode, and MchromCode.

The NcdfGenotypeReader constructor creates and returns a NcdfGenotypeReader instance pointing to this file.

Accessors

In the code snippets below, object is a NcdfGenotypeReader object.

See NcdfReader for additional methods.

nsnp(object): The number of SNPs in the NetCDF file.

nscan(object): The number of scans in the NetCDF file.

getSnpID(object, index): A unique integer vector of snp IDs. The optional index is a logical or integer vector specifying elements to extract.

getChromosome(object, index, char=FALSE): A vector of chromosomes. The optional index is a logical or integer vector specifying elements to extract. If char=FALSE (default), returns an integer vector. If char=TRUE, returns a character vector with elements in (1:22,X,XY,Y,M,U). "U" stands for "Unknown" and is the value given to any chromosome code not falling in the other categories.

getPosition(object, index): An integer vector of base pair positions. The optional index is a logical or integer vector specifying elements to extract.

getScanID(object, index): A unique integer vector of scan IDs. The optional index is a logical or integer vector specifying elements to extract.

getGenotype(object, snp=c(1,-1), scan=c(1,-1), drop=TRUE, use.names=FALSE, ...): Extracts genotype values (number of A alleles). snp and scan indicate which elements to return along the snp and scan dimensions. They must be integer vectors of the form (start, count), where start is the index of the first data element to read and count is the number of elements to read. A value of '-1' for count indicates that the entire dimension should be read. If drop=TRUE, the result is coerced to the lowest possible dimension. If use.names=TRUE and the result is a matrix, dimnames are set to the SNP and scan IDs. Missing values are represented as NA.

getVariable(object, varname, ...): Extracts the contents of the variable varname. If the variable is not found in the NetCDF file, returns NULL.

autosomeCode(object): Returns the integer codes for the autosomes.

XchromCode(object): Returns the integer code for the X chromosome.

XYchromCode(object): Returns the integer code for the pseudoautosomal region.

YchromCode(object): Returns the integer code for the Y chromosome.

MchromCode(object): Returns the integer code for mitochondrial SNPs.

Author(s)

Stephanie Gogarten

See Also

NcdfReader, NcdfIntensityReader, GenotypeData, IntensityData

Examples

file <- system.file("extdata", "illumina_geno.nc", package="GWASdata")
nc <- NcdfGenotypeReader(file)

# dimensions
nsnp(nc)
nscan(nc)

# get snpID and chromosome
snpID <- getSnpID(nc)
chrom <- getChromosome(nc)

# get positions only for chromosome 22
pos22 <- getPosition(nc, index=(chrom == 22))

# get all snps for first scan
geno <- getGenotype(nc, snp=c(1,-1), scan=c(1,1))

# starting at snp 100, get 10 snps for the first 5 scans
geno <- getGenotype(nc, snp=c(100,10), scan=c(1,5))

close(nc)

amstilp/GWASTools documentation built on May 10, 2019, 1:08 a.m.