Samples_Per_Lane: Estimate the number of samples per single Illumina lane

Description Usage Arguments Value Author(s) See Also Examples

Description

This function calculates the estimated maximum number of samples a user can multiplex in one Illumina lane given the number of cutsites, while maintaining a desired user-defined coverage.

Usage

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Samples_Per_Lane(num_cutsites,
                 desired_coverage = NULL,
                 sequencing_machine = NULL)

Arguments

num_cutsites

Number of cutsites present in the genome. This number can be the output from number_cutsites()

desired_coverage

Number of the coverage the user wishes to keep. If no value is provided, the default value is 30.

sequencing_machine

Illumina sequencing platform that the user wants to use for sequencing. Argument has two parameters, hiseq2500 and hiseq4000, each has different read throughputs. If no machine is provided, the function will default to hiseq2500.

Value

Returns a vector with an estimate of low, medium, and high estimates of the number of samples a user can fit in a single Illumina lane.

Author(s)

Kira Long

See Also

number_cutsites

Examples

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#Define parameters
numberCuts <- 39000
coverage <- 30

#Run arguments in Function
Samples_Per_Lane(numberCuts,
                 coverage,
                 "hiseq4000")

angelgr2/radseq_tools documentation built on May 15, 2019, 3:59 a.m.